Pochettino's famous demands on his players will be put to the test again at Wembley with the Argentine expecting maximum effort to stop his compatriot Messi who scored a hat-trick in Barca's 4-0 win over PSV last month.
At the club's open training session on Tuesday, a host of top names were nowhere to be seen - Dele Alli, Jan Vertonghen, Mousa Dembele, Christian Eriksen, Serge Aurier and Michel Vorm.
Spurs lost their opening match of the competition against Inter at San Siro, despite leading the match 1-0 with six minutes to play.
Cristiano Ronaldo and Messi continue to set the bar, and Kane believes that's a motivating factor.
So the North London giants will be heading into Wembley's crunch Champions League clash with Barca on Wednesday with renewed confidence.
"He's definitely set the standard for the rest of us".
Pochettino accepts Messi will be Barcelona's biggest threat.
Messi's response according to the report was to attack Barcelona's defense stating that they are conceding too many goals this season. "It is a period when we are not in good luck with injuries... It's only about enjoying it, being close, helping the players that are going to face him to stay close and, when you have the ball, trying to have possession, to play in the opposite half, to try to push Leo to play far from your goal".
Barcelona coach, Ernesto Valverde will be without defender, Sergi Roberto, who sustained an injury against Athletic, while Samuel Umtiti is both injured and suspended.