Appearing nervous and uncomfortable, the men confirmed their names as those announced by the United Kingdom investigators - Alexander Petrov and Ruslan Boshirov.
The editor in chief was quoted by another television station in Russian Federation as saying to her surprise the two spoke in some detail about their work and how they earn their living.
He and his daughter Yulia became seriously ill, while a police officer on the case was also hospitalized.
In the men's first interview since they were named publicly they denied carrying women's perfume, after police discovered a counterfeit bottle that contained a "significant amount" of Novichok.
Challenging Alexander Petrov and Ruslan Boshirov, the Tory MP said: "We had this outrageous event in Salisbury, Wiltshire, when two agents of the GRU were involved in the murder of Dawn Sturgess and attempted murder of others".
The suspected assassins' alibi of tourists visiting the "123 metre spire" of the town's cathedral also sparked incredulity.
May spokeswoman Alison Donnelly told reporters Thursday the United Kingdom remains confident "These men are officers of the Russian military intelligence service who used a devastatingly toxic chemical weapon on the streets of our country".
They also asked for an apology from the United Kingdom authorities, adding: "We just want this to be over".
Downing Street, meanwhile, dismissed the claims by the two men as "lies and blatant fabrications".
The UK Crown Prosecution Service (CPS) says there is enough evidence to convict the two men, although it is not applying to Russian Federation for their extradition because Russian Federation does not extradite its own nationals.
The full unedited interview still hasn't been published online but the RT anchors addressed the obvious question of why these two men would approach RT, a cable network whose audience is primarily people outside of Russian Federation.
"On Saturday, 3 March, they left the hotel and took the underground to Waterloo station, arriving at approximately 11.45am, where they caught a train to Salisbury, arriving at approximately 2.25pm". Petrov claimed they were trying to go for a walk on the 3rd, "but we lasted for only half an hour because it was covered in snow".
"From the very start we planned to go to London and, put bluntly, cut loose and have some fun", he said in the interview which was pre-recorded on Wednesday and broadcast on Thursday.
Vladimir Putin, expressing his views in the matter at an economic forum in the Russian Pacific port city of Vladivostok, said Russia had located the two men, but there nothing surreal about it. "Why would a man have perfume for women in his luggage?"
"They are civilians", Putin said, adding there was nothing criminal about them. They then made a decision to return the next day.
The men, who appeared to be about 40, claimed they did not know who Skripal was or where he lived.
"I'm frightened, I'm scared".
Both men looked composed during the interview, but Boshirov said, "We fear for our lives".
"Accusing Russia of lying after the declarations of two Russian citizens is. absurd", Kremlin spokesman Dmitry Peskov said.
British officials decried the interview, calling it "risible".
"It could be a way of signalling defiance to the United Kingdom government, effectively saying, "There's nothing you can do about this", he said. "And the CCTV coverage as well... we didn't see any footage of the Skripals on the day of the poisoning", he told RT.
"I'll say it now - those two guys on Russian TV are murderers".