"The 10-year veteran officer resigned late Wednesday, according to Cook County Forest Preserve District officials, rather than fight to keep his badge over the encounter that has been recorded and viewed millions of times, drawing global scorn for Connor's inaction", the outlet reported.
Forest preserve officials said they "are further addressing aspects of this incident" and more details are forthcoming.
He was first charged with the misdemeanor after he was caught on camera accosting Mia Irizarry at a Cooks County Forest Preserve picnic area in June.
Mia Irizarry says she was trying to celebrate her 24th birthday in the Forest Preserves of Cook County last month when the man approached her asking her why she was wearing the sleeveless Puerto Rico flag shirt, which also had "Puerto Rico" written below the V neckline.
"You're not American, if you were American you wouldn't wear that".
During the clip, which went on to be viewed almost two million times, Trybus yells at Irizarry that she "should not be wearing that in the United States of America" in reference to her Puerto Rican flag shirt, as well as repeatedly asking if she is an American citizen. Irizarry is heard telling Trybus that Puerto Rico is part of the United States as he approaches her multiple times.
That's a central fear among white nationalists, the alt-right, racists or whatever you want to call them: Immigrants are coming to America and fundamentally changing the country, moving it away from what they believe are its white, European roots.
Ms Irizarry calls on a nearby park police officer for help. When the new officers arrived, they arrested Trybus on charges of misdemeanor assault and misdemeanor disorderly conduct. She said it took two hours before officers arrested Trybus for disorderly conduct.
One of the other responding officers tells Trybus that he can't be harassing people, adding that people have just as much right to be in the park as he does.
At a press conference on Thursday, Superintendent Arnold Randall said Mr Connor was "very remorseful" when he resigned.
It's not clear when the investigation into the officer's response will be completed.
The video of the exchange sparked outrage, and even Puerto Rico's governor, Ricardo Rosselló, weighed in and called it "appalling" and he said "a Puerto Rican woman was brutally harassed by a bigot while an officer did not interfere".
"The investigation is ongoing and the officer involved has been assigned to desk duty pending the outcome". Officer Connor's actions came under intense fire based on the video. "We still don't know what happened outside the video". We can not allow those who do not understand America's greatness to terrorize people due to their background. He also questions her about whether she was an American citizen.
Meanwhile, the Forest Preserves issued its own apology for what happened.