The gathering comes as America, Britain and France yesterday launched a coordinated airstrike in Syria to punish the regime for a chemical weapons attack that killed more than 70 people.
The Syrian government denies using or possessing chemical weapons and said the strikes were an act of aggression.
The heads of Arab states will renew their rejection of US President Donald Trump's decision to recognise Jerusalem as the capital of Israel and to relocate the US embassy from Tel Aviv to Jerusalem, slated for May.
Iranian Foreign Ministry Spokesman Bahram Qassemi made the remarks early on Monday in response to the final communique of Arab League's 29th summit.
The summit will offer an opportunity for Arab leaders to hold smaller meetings on the sidelines to discuss and coordinate on a wide range of issues, including how to move forward on Syria, how to handle the tense situation in Gaza, and what to do about Libya.
The statement also included the need to confront regional intervention in the domestic affairs of Arab countries, in addition to any intervention that targets their social unity and national identity.
Ahmed Aboul Gheit, secretary-general of the Arab League, said that the serious crises in the region facilitated foreign interference.
In attendance were leaders from the Middle East and North Africa, including Egyptian President Abdel Fattah al-Sisi and Jordanian King Abdullah.
Riyadh expressed its support for the strikes on Damascus in a statement on Saturday.
Mr Trump then told reporters that the relationship between the two countries was "probably the strongest it has ever been", adding: "We understand each other". The kingdom announced another $50 million for programs run by the United Nations relief agency for Palestinians after the US slashed its aid.
But King Salman avoided any mention of Syria in his address, as a seat marked "Syrian Arab Republic" sat empty in the hall.
Tensions have eased slightly in recent months but Qatar still only sent its representative to the Arab League to the Dhahran summit. While the Qatari flag was erected alongside other member-state flags on the streets of Dhahran, the country's representative did not appear in a group photo of the top delegates in attendance.
The rift between Qatar, Saudi Arabia, the UAE and Bahrain will not be on the summit's agenda.
Iran has dismissed the allegations that it supports the Yemeni rebels.