Eric Schneiderman filed the civil rights suit against the Weinstein Company, claiming it repeatedly broke NY law by failing to protect its employees from pervasive sexual harassment, intimidation and discrimination.
The lawsuit has halted a potential sale of the company.
The lawsuit alleges that the company broke NY law and violated the company's employees' rights by "failing to protect its employees from pervasive sexual harassment, intimidation, and discrimination", Schneiderman said in a statement.
Schneiderman's office launched an investigation in October into the brothers' company as accusations of sexual harassment and assault against Harvey Weinstein - and a subsequent cover up - began to surface.
The Attorney General's lawsuit alleges that company executives and board members repeatedly failed to protect employees from their then-CEO's unrelenting sexual harassment, intimidation, and discrimination.
USA TODAY has reached out to representatives for The Weinstein Company for comment.
State attorney general Eric Schneiderman said the lawsuit was filed in New York County Supreme Court and included new allegations about Weinstein's "vicious and exploitative mistreatment" of employees.
In one case, the probe found that "in a fit of rage against one female employee, he yelled that she should leave the company and make babies since that was all she was good for". He has denied all allegations of non-consensual sex, as well as claims that he retaliated against women who rebuffed him.